The octagonal Chapel attributed to Sanmicheli is situated in the north-east corner of the upper courtyard, bordering with the road. Apparently a minor structure, it in fact a scenic backdrop to the short pathway to the Villa. The central structure is octagonal, with irregular sides and four large apsidal niches and alternating minor niches with windows above them along the short diagonal sides. The internal overhead structure with its domed ceiling and roof - entirely free of decorative elements or moldings – is concealed on the outside by the continuous perimeter wall of the complex.
The location of the chapel at a distance from the main body of the Villa, flanking the road and facing the main entrance to the complex, suggests it may have had both a public as well as a private religious function.